Why R/mrgsolve?

  • Fun!

What is PBPK modeling?

A bottom-up modeling approach that uses physiological parameters to describe a drug’s pharmacokinetics. The concept of PBPK modeling was introduced by Torsten Teorell in 1937 https://www.tandfonline.com/doi/pdf/10.3109/03009739509178895

Difference between popPK and PBPK modeling

https://www.mdpi.com/1999-4923/9/4/41/htm

  • Advantages of PBPK:
    • Bottom-up approach that can be used in case of little to no clinical data
      • special populations
      • environmental toxicology
    • Provides a mechanistic explanation of patient variability
    • Inter- and intra-species scalability
    • Well suited for DDI modeling
    • Provides drug concentrations in organs not accurately represented by plasma concentration (eye, skin, etc..)
  • Disadvantages of PBPK:
    • Complex
    • Computationally intensive

General PBPK Workflow

Let’s Start Coding..

1. Simple PBPK Model

# load libraries
library(tidyverse)
library(mrgsolve)

a. Model structure

b. Equations

  • Non-eliminating compartment (Muscle)

\[\frac{dA_{mu}}{dt}=Q_{mu}(C_{art}-\frac{C_{mu}}{\frac{Kp_{mu}}{BP}})\]

  • Eliminating compartment (Liver):

\[\frac{dA_{li}}{dt}=Q_{li}(C_{art}-\frac{C_{li}}{\frac{Kp_{li}}{BP}})-Cl_{li}.f_{u}.\frac{C_{li}}{\frac{Kp_{li}}{BP}}\]

  • Arterial blood compartment:

\[\frac{dA_{art}}{dt}=Q_{lu}(\frac{C_{lu}}{\frac{Kp_{lu}}{BP}}-C_{art})\]

  • Venous blood compartment:

\[\frac{dA_{ven}}{dt}=\sum_{T\neq lu} (Q_T.\frac{C_T}{\frac{Kp_T}{BP}}) − Q_{lu}.C_{ven}\]

  • Lungs:

\[\frac{dA_{lu}}{dt}=Q_{lu}(C_{ven} − \frac{C_{lu}}{\frac{Kp_{lu}}{BP}})\]

c. Tasks

  1. Use models/simplePBPK.mod file to build a simple PBPK model.

  2. Use script.R script to compile the model and run a simple simulation (chunks 1 and 2).

2. Voriconazole PBPK Model

a. References

Zane NR, Thakker DR. A physiologically based pharmacokinetic model for voriconazole disposition predicts intestinal first-pass metabolism in children. Clin Pharmacokinet. 2014;53: 1171–1182
https://link.springer.com/article/10.1007%2Fs40262-014-0181-y

ICRP Publication 89 http://www.icrp.org/publication.asp?id=ICRP%20Publication%2089

Utsey, Kiersten, Madeleine S. Gastonguay, Sean Russell, Reed Freling, Matthew M. Riggs, and Ahmed Elmokadem. 2020. “Quantification of the Impact of Partition Coefficient Prediction Methods on PBPK Model Output Using a Standardized Tissue Composition.” Drug Metabolism and Disposition: The Biological Fate of Chemicals, July. https://doi.org/10.1124/dmd.120.090498.

b. Model structure

c. Tasks

Note: for the following tasks use script.R

  1. Explore and compile typical adult male PBPK model models/voriPBPK.mod
modA <- mread("models/voriPBPK.mod")
  1. Use calcKp_PT.R function to calculate voriconzole tissue:plasma partition coefficients according to Poulin and Theil method https://www.ncbi.nlm.nih.gov/pubmed/11782904.
source("calcKp_PT.R")

#voriconazole physicochemical properties
logP <- 2.56  #lipophilicity
pKa <- 1.76  
fup <- 0.42   #unbound fraction in plasma
type <- 3     #monoprotic base
BP <- 1       #blood:plasma concentration ratio
dat <- read.csv("data/tissue_comp_PT.csv")

#calculate partition coefficients
Kp <- calcKp_PT(logP=logP, pKa=pKa, fup=fup, BP=BP, type=type, dat=dat)

#update model parameters partition coefficients
modA <- param(modA, Kp)
  1. Simulate the steady state after a 4 mg/kg IV infusion dose of voriconazole given to an adult male with a rate of 4 mg/kg/h twice a day for 7 days. Compare the steady state plasma drug concentration-time profiles from previous simulation to the observed data in Adult_IV.csv. (N.B.: observed data were digitized from Zane and Thakker (2014) paper using WebPlotDigitizer https://automeris.io/WebPlotDigitizer/):
#load observed IV infusion data
obs <- read.csv("data/Adult_IV.csv")

#set simulation conditions
bw   <- 73
amt  <- 4*bw
rate <- 4*bw
cmt  <- "VEN"
ii   <- 12
addl <- 13
ss   <- 1

#run simulation
sim <- 
  modA %>% 
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=ss) %>% 
  mrgsim(delta = 0.1, end = 12) %>% 
  filter(row_number() != 1)  

#plot prediction and compare to observed data
gp <- ggplot() + 
  geom_point(data = obs, aes(x=time, y=obs, col="observed"), size=2.5) + 
  geom_errorbar(data = obs, aes(x = time, y = obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data = sim, aes(x=time, y=CP, col="sim"), lwd=1) + 
  scale_colour_manual(name='', 
                      values=c('sim'='black', 'observed'='black'), 
                      breaks=c("observed","sim"),
                      labels=c("observed","predicted")) +
  guides(colour = guide_legend(override.aes = list(linetype=c(0,1), shape=c(16, NA)))) +
  labs(title="Adult 4 mg/kg IV", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

  1. Simulate the steady state after a 200 mg PO dose of voriconazole given to an adult male with a rate of twice a day for 7 days. Compare the steady state plasma drug concentration-time profile to the observed data in Adult_PO.csv.
obs <- read.csv("data/Adult_PO.csv")

bw   <- 73
amt  <- 200
cmt  <- "GUTLUMEN"
ii   <- 12
addl <- 13
ss   <- 1

sim <- 
  modA %>% 
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, ss=ss) %>% 
  mrgsim(delta = 0.1, end = 12) %>% 
  filter(row_number() != 1)  

gp <- ggplot() + 
  geom_point(data = obs, aes(x=time, y=obs, col="observed"), size=2.5) + 
  geom_errorbar(data = obs, aes(x = time, y = obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data = sim, aes(x=time, y=CP, col="sim"), lwd=1) + 
  scale_colour_manual(name='', 
                      values=c('sim'='black', 'observed'='black'), 
                      breaks=c("observed","sim"),
                      labels=c("observed","predicted")) +
  guides(colour = guide_legend(override.aes = list(linetype=c(0,1), shape=c(16, NA)))) +
  labs(title="Adult 4 mg/kg PO", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

  1. Integrate the model with an additional gut wall enterocyte compartment to account for intestinal clearance, intestinal transit and lumen solubility effects on absorption rate. Note: use about an intestinal clearance that is 30 times lower than hepatic clearance. Recompile and re-run the previous step. Any change!!

modA <- mread("models/voriPBPK_ext.mod") %>%
  param(MPPGI = 30.3/30) %>%
  param(Kp)

sim <- 
  modA %>% 
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, ss=ss) %>% 
  mrgsim(delta = 0.1, end = 12) %>% 
  filter(row_number() != 1)  

gp <- ggplot() + 
  geom_point(data = obs, aes(x=time, y=obs, col="observed"), size=2.5) + 
  geom_errorbar(data = obs, aes(x = time, y = obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data = sim, aes(x=time, y=CP, col="sim"), lwd=1) + 
  scale_colour_manual(name='', 
                      values=c('sim'='black', 'observed'='black'), 
                      breaks=c("observed","sim"),
                      labels=c("observed","predicted")) +
  guides(colour = guide_legend(override.aes = list(linetype=c(0,1), shape=c(16, NA)))) +
  labs(title="Adult 4 mg/kg PO", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

  1. Generate pediatric model
# pediatric (5 yo) male physiology; https://www.ncbi.nlm.nih.gov/pubmed/14506981
pedPhys <- list(WEIGHT = 19,
                Vad = 5.5,
                Vbo = 2.43,
                Vbr = 1.31,
                VguWall = 0.22,
                VguLumen = 0.117,
                Vhe = 0.085,
                Vki = 0.11,
                Vli = 0.467,
                Vlu = 0.125,
                Vmu = 5.6,
                Vsp = 0.05,
                Vbl = 1.5,
                Qad = 0.05*3.4*60,
                Qbo = 0.05*3.4*60,
                Qbr = 0.12*3.4*60,
                Qgu = 0.15*3.4*60, 
                Qhe = 0.04*3.4*60,
                Qki = 0.19*3.4*60,
                Qmu = 0.17*3.4*60,
                Qsp = 0.03*3.4*60,
                Qha = 0.065*3.4*60, 
                Qlu = 3.4*60,
                MPPGL = 26,
                VmaxH = 120.5,
                KmH = 11,
                MPPGI = 0,
                VmaxG = 120.5,
                KmG = 11)

modP <- param(modA, pedPhys)
  1. Simulate the steady state after a 4 mg/kg voriconazole IV infusion dosing in a male child subject infused at a rate of 3 mg/kg/h twice a day for seven days. Compare the steady state to the observed data in Pediatric_IV.csv.
obs <- read.csv("data/Pediatric_IV.csv")  #load observed data

wt   <- 19  #pediatric body weight
amt  <- 4*wt  
rate <- 3*wt
cmt  <- "VEN"  #intravenous infusion
ii   <- 12
addl <- 13
ss   <- 1

# simulate
sim <- 
  modP %>%
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=1) %>% 
  mrgsim(delta = 0.1, end = 12) %>% 
  dplyr::filter(row_number() != 1)  

# plot
gp <- ggplot() + 
  geom_point(data = obs, aes(x=time, y=obs, col="observed"), size=2.5) + 
  geom_errorbar(data = obs, aes(x = time, y = obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data = sim, aes(x=time, y=CP, col="sim"), lwd=1) + 
  scale_colour_manual(name='', 
                      values=c('sim'='black', 'observed'='black'), 
                      breaks=c("observed","sim"),
                      labels=c("observed","predicted")) +
  guides(colour = guide_legend(override.aes = list(linetype=c(0,1), shape=c(16, NA)))) +
  labs(title="Pediatric 4 mg/kg IV", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

  1. Simulate the steady state after a 4 mg/kg voriconazole PO dosing in a male child subject twice a day for seven days. Compare to obsreved data in Pediatric_PO.csv Note: Include a similar 30-fold lower intestinal clearance than hepatic clearance.
obs <- read.csv("data/Pediatric_PO.csv")  #load observed data

# adjust intestinal clearance
modP <- modP %>% param(MPPGI = 26 / 30)

# simulation conditions
bw   <- 19
amt  <- 4 * bw
cmt  <- "GUTLUMEN"
ii   <- 12
addl <- 13
ss   <- 1

# simulate
sim <- 
  modP %>%
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, ss=1) %>% 
  mrgsim(delta = 0.1, end = 12) %>% 
  dplyr::filter(row_number() != 1)  

# plot
gp <- ggplot() + 
  geom_point(data = obs, aes(x=time, y=obs, col="observed"), size=2.5) + 
  geom_errorbar(data = obs, aes(x = time, y = obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data = sim, aes(x=time, y=CP, col="sim"), lwd=1) + 
  scale_colour_manual(name='', 
                      values=c('sim'='black', 'observed'='black'), 
                      breaks=c("observed","sim"),
                      labels=c("observed","predicted")) +
  guides(colour = guide_legend(override.aes = list(linetype=c(0,1), shape=c(16, NA)))) +
  labs(title="Pediatric 4 mg/kg PO", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

d. Sensitivity Analysis

i. Local

Graphical

Run graphical sensitivity analysis for the muscle:plasma (Kpmu) and lung:plasma (Kplu) partition coefficients using adult IV data.

#load observed IV infusion data
obs <- read.csv("data/Adult_IV.csv")

#set simulation conditions
bw   <- 73
amt  <- 4*bw
rate <- 4*bw
cmt  <- "VEN"
ii   <- 12
addl <- 13
ss   <- 1

##' Define an intervention
e <- ev(cmt=cmt, amt=amt, rate=rate, ii= ii, addl=addl, ss=1)

## Sensitivity analysis on Kpmu
idata <- expand.idata(Kpmu = c(3/2, 3, 3*2))

modA %>%
  carry_out(Kpmu) %>% 
  mrgsim_ei(e, idata, delta = 0.1, recsort=3, obsonly=TRUE, end = 12) %>% 
  mutate(Kpmu = factor(Kpmu)) %>%
  ggplot(aes(x=time, y=CP, col=Kpmu)) +
  geom_line() +
  labs(x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()

##' Sensitivity analysis on Kplu
idata <- expand.idata(Kplu = c(1/2, 1, 1*2))

modA %>%
  carry_out(Kplu) %>% 
  mrgsim_ei(e, idata, delta = 0.1, recsort=3, obsonly=TRUE, end = 12) %>% 
  mutate(Kplu = factor(Kplu)) %>%
  ggplot(aes(x=time, y=CP, col=Kplu)) +
  geom_line() +
  labs(x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()

mrgsim.sa package

The package mrgsim.sa simplifies the process of running local sensitivity analysis https://github.com/kylebaron/mrgsim.sa. The package can do graphical sensitivity as well as obtaining local sensitivity coefficients.

\[\frac{\partial{y_i}}{\partial{\theta_j}}.\frac{w_{\theta{j}}}{w_{y_i}}\]

What is the most influential parameter?

library(mrgsim.sa)

### sensitivity analysis
#set a list of the candidate parameters
#parms <- list(Kpad = 9.89, Kpbo = 7.91, Kpbr = 7.35, Kpgu = 5.82, Kphe = 1.95, Kpki = 2.9, Kpli = 4.66, Kplu = 0.83, Kpmu = 2.94, Kpsp = 2.96) 

#set the output variable of interest
sensvar <- c("CP")  

# graphical sensitivity for each parameter
out_sens <- 
  modA %>% 
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=ss) %>% 
  select_par(all_of(names(Kp[-11]))) %>% 
  parseq_fct(.n=3) %>% 
  sens_each(delta = 0.1, recsort=3, obsonly=TRUE, end = 12)

sens_plot(out_sens, "CP", grid = TRUE) 

# graphical sensitivity for a grid of parameters
out_sens_grid <- 
  modA %>% 
  ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=ss) %>% 
  parseq_cv(Kpmu, Kplu, .n=3) %>% 
  sens_grid(delta = 0.1, recsort=3, obsonly=TRUE, end = 12)

sens_plot(out_sens_grid, "CP")

# local sensitivity analysis 
out_lsa <- lsa(modA, var = "CP", par = names(all_of(Kp[-11])), events = ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=ss), end=12, delta=0.1, eps=1e-4)

lsa_plot(out_lsa, pal = NULL)

# get a summary of sensitivity coefficients
out_lsa_summ <- out_lsa %>% 
  group_by(p_name) %>%
  summarise(mean_sens = mean(sens)) %>%
  ungroup()

ggplot(data=out_lsa_summ, aes(x=reorder(p_name, mean_sens), y=mean_sens)) + 
    geom_col() + 
    labs(x="Parameter", y="Coefficient") +
    coord_flip() +
    geom_hline(yintercept = 0, lty=2) +
    theme_bw()

ii. Global

Review sobol global sensitivity analysis from sensitivity package (https://cran.r-project.org/web/packages/sensitivity/index.html)
Zhang, X-Y, M. N. Trame, L. J. Lesko, and S. Schmidt. 2015. “Sobol Sensitivity Analysis: A Tool to Guide the Development and Evaluation of Systems Pharmacology Models.” CPT: Pharmacometrics & Systems Pharmacology 4 (2): 69–79. https://ascpt.onlinelibrary.wiley.com/doi/full/10.1002/psp4.6
Vignette: https://github.com/mrgsolve/gallery/blob/master/application/sobol.md

e. Parameter Estimation

library(nloptr)
library(numDeriv)
library(kableExtra)

### optimization with maximum likelihood method
theta <- log(c(Kpmu=2.94, sigma=1))  #initial parameter for MLE; mean and variance
sampl <- obs$time                    #sampling times from observed data

bw <- 73
amt = 4*bw
rate = 4*bw
cmt = "VEN"
ii = 12
addl = 13
ss = 1

##set up objective function
OF <- function(pars, pred=F){
  pars <- lapply(pars,exp)  #Get out of log domain for MLE
  pars <- as.list(pars)
  names(pars) <- names(theta)
  
  ## Get a prediction
  out <- as.data.frame(modA %>% 
                         param(pars) %>%
                         ev(cmt=cmt, amt=amt, rate=rate, ii=ii, addl=addl, ss=ss) %>%
                         mrgsim(end=-1, add=sampl)
  )
  
  out <- out[-1,] 
  
  if(pred) return(out)
  
  ##OLS
  #return(sum((out$Cvenous - obs$obs)^2))
  
  ##maximum likelihood
  return(-1*sum(dnorm(log(obs$obs),
                      mean=log(out$CP),
                      sd=pars$sigma, log=TRUE)))
}

##Fit with nloptr package
##derivative-free optimizers
#fit <- neldermead(theta, OF)  #Nelder-Mead simplex
fit <- newuoa(theta, OF)  #New Unconstrained Optimization with quadratic Approximation

##gradient-basd optimizers
#fit <- tnewton(theta, OF)  #Local optimizer; Nelder-Mead simplex
#fit <- mlsl(theta, OF, lower=log(c(0.1)), upper=log(c(10)))  #global optimizer; multi-level single-linkage; takes very long ~ 10 min

##global optimizers
#fit <- direct(OF, lower=log(c(0.1, 0.1)), upper=log(c(10, 2)))  #DIviding RECTangles algorithm; takes ~ 3 min

fit
## $par
## [1] -0.5708576 -2.0482601
## 
## $value
## [1] -8.181457
## 
## $iter
## [1] 52
## 
## $convergence
## [1] 1
## 
## $message
## [1] "NLOPT_SUCCESS: Generic success return value."
p <- as.list(exp(fit$par))  #get the parameters on the linear scale
names(p) <- names(theta)
p
## $Kpmu
## [1] 0.5650407
## 
## $sigma
## [1] 0.1289591
# get standard error and confidence intervals around the estimated parameters
h <- hessian(OF, fit$par)
vc_log <- solve(h)  #variance-covariance matrix
SE_log <- sqrt(diag(vc_log))  #standard error on log scale

# create dataframe with parameter summary
sig <- function(x) signif(x, 3)
paramSumm <- tibble(Parameter = names(theta),
                    Estimate = exp(fit$par),
                    lb = exp(fit$par - (1.96 * SE_log)),
                    ub = exp(fit$par + (1.96 * SE_log))) %>%
  mutate(`Estimate (95% CI)` = paste0(sig(Estimate), " (", sig(lb), ", ", sig(ub), ")")) %>%
  select(Parameter, `Estimate (95% CI)`)

paramSumm %>%
  knitr::kable() %>%
  kable_styling()
Parameter Estimate (95% CI)
Kpmu 0.565 (0.201, 1.59)
sigma 0.129 (0.0878, 0.189) 
# compare initial predictions to those using the optimized parameters
predB4 <- OF(theta, pred=T)
predAfter <- OF(fit$par, pred=T)

gp <- ggplot() +
  geom_point(data=obs, aes(x=time, y=obs)) + 
  geom_errorbar(data=obs, aes(x=time, y=obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data=predB4, aes(x=time, y=CP), lty=2) +
  geom_line(data=predAfter, aes(x=time, y=CP)) +
  labs(x="time (h)", y="Plasma concentration (mg/L)") + 
  theme_bw()
gp

check out:

Simulate a 4 mg/kg voriconazole IV infusion dosing in a male child subject infused at a rate of 3 mg/kg/h twice a day for seven days. Compare the steady state prediction before and after optimization to the observed data in Pediatric_IV.csv.

obs <- read.csv("data/Pediatric_IV.csv")  #load observed data

wt <- 19  #adult body weight
amt <- 4*wt  
rate <- 3*wt
cmt <- "VEN"  #intravenous infusion
ii = 12
addl = 13
ss = 1

# simulate
simB4 <- as.data.frame(modP %>%
                         ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=1) %>% 
                         mrgsim(delta = 0.1, end = 12)) %>% 
  dplyr::filter(row_number() != 1)  

simAfter <- as.data.frame(modP %>%
                            param(Kpmu=p$Kpmu) %>%
                            ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=1) %>% 
                            mrgsim(delta = 0.1, end = 12)) %>% 
  dplyr::filter(row_number() != 1)

# plot
gp <- ggplot() + 
  geom_point(data = obs, aes(x=time, y=obs), size=2.5) + 
  geom_errorbar(data = obs, aes(x = time, y = obs, ymin=obs-sd, ymax=obs+sd), width=0) +
  geom_line(data = simB4, aes(x=time, y=CP), lty=2) + 
  geom_line(data = simAfter, aes(x=time, y=CP)) + 
  labs(title="Pediatric 4 mg/kg IV", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

f. Population simulations

i. How to create a population?

Need to introduce interindividual variability to physiological parameters

Willmann, Stefan, Karsten Höhn, Andrea Edginton, Michael Sevestre, Juri Solodenko, Wolfgang Weiss, Jörg Lippert, and Walter Schmitt. 2007. “Development of a Physiology-Based Whole-Body Population Model for Assessing the Influence of Individual Variability on the Pharmacokinetics of Drugs.” Journal of Pharmacokinetics and Pharmacodynamics 34 (3): 401–31. https://pubmed.ncbi.nlm.nih.gov/17431751/

Huisinga, W., A. Solms, L. Fronton, and S. Pilari. 2012. “Modeling Interindividual Variability in Physiologically Based Pharmacokinetics and Its Link to Mechanistic Covariate Modeling.” CPT: Pharmacometrics & Systems Pharmacology 1 (September): e4. https://ascpt.onlinelibrary.wiley.com/doi/10.1038/psp.2012.3

“NHANES - National Health and Nutrition Examination Survey Homepage.” 2018. July 26, 2018. http://www.cdc.gov/nchs/nhanes/.

Meyer, Michaela, Sebastian Schneckener, Bernd Ludewig, Lars Kuepfer, and Joerg Lippert. 2012. “Using Expression Data for Quantification of Active Processes in Physiologically Based Pharmacokinetic Modeling.” Drug Metabolism and Disposition: The Biological Fate of Chemicals 40 (5): 892–901. https://pubmed.ncbi.nlm.nih.gov/22293118/

ii. Simulation

  1. Simulate a simple voriconazole dosing scenario for a population of 100 individuals, 50 females and 50 males, with ages ranging between 20-80, weights ranging between 50-100 kg and heights between 1.5 and 1.9 m. Use population saved in file ../data/derived/popPars_100.rds
# load population params
popPars <- readRDS("data/popPars_100.rds")

# add IIV on ka and CL/VmaxH
set.seed(192898)
iVmaxH <- rlnorm(100, meanlog=log(40), sdlog=0.2)

# add to popPars
popPars2 <- lapply(1:length(popPars), function(i){
  pars <- c(popPars[[i]],list(VmaxH = iVmaxH[i]))
  return(pars)
  })

# simulate
modA2 <- mread("models/voriPBPK2.mod")
Kpmu <- 0.56
modA2 <- param(modA2, Kpmu=Kpmu)

#set simulation conditions
bw   <- 73
amt  <- 4*bw
rate <- 4*bw
cmt  <- "VEN"
ii   <- 12
addl <- 13
ss   <- 1
delta <- 0.1
end <- 12

# prepare simulation dataset
idata <- popPars2 %>% bind_rows()

e <- ev(amt=amt, cmt=cmt, ii=ii, addl=addl, rate=rate, ss=ss)

data <- e %>% 
  as_tibble %>% 
  bind_cols(idata) %>%
  mutate(amt = 4*BW, rate = 4*BW) %>%
  select(ID, everything())

#run simulation
system.time(sims <- modA2 %>% 
  mrgsim_d(data=data, delta = delta, end = end, obsonly=T, outvars = c("CP")) %>% 
  filter(row_number() != 1))
##    user  system elapsed 
##   8.723   0.000   8.723
# get summary stats for population prediction
hi95 <- function(x) quantile(x, probs = c(0.95))
lo05 <- function(x) quantile(x, probs = c(0.05))

sims2 <- sims %>%
  group_by(time) %>%
  mutate(loCP = lo05(CP),
         medCP = median(CP),
         hiCP = hi95(CP)) %>%
  ungroup() %>%
  filter(ID == first(ID))

#plot population predictions 
gp <- ggplot(data = sims2, aes(x=time)) + 
  geom_line(aes(y=medCP), col="black") +
  geom_ribbon(aes(ymin=loCP, ymax=hiCP), alpha = 0.5) +
  scale_y_continuous(trans = "log10") +
  labs(title="Population simulation", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

  1. Use PKNCA package to calculate summary statistics for the population
library(PKNCA)

# create dose object
dose_obj <-
  PKNCAdose(
    data,
    amt~time|ID
  )

# create conc object
conc_obj <-
  PKNCAconc(
    sims,
    CP~time|ID
  )

# combine
data_obj <- PKNCAdata(conc_obj, dose_obj)

## Calculate the NCA parameters
results_obj <- pk.nca(data_obj)

## Summarize the results
summary(results_obj)
##  start end   N     auclast        cmax              tmax   half.life
##      0  24 100 26.2 [19.6]           .                 .           .
##      0 Inf 100           . 5.33 [16.7] 1.00 [1.00, 1.00] 27.7 [7.44]
##   aucinf.obs
##            .
##  86.5 [42.6]
## 
## Caption: auclast, cmax, aucinf.obs: geometric mean and geometric coefficient of variation; tmax: median and range; half.life: arithmetic mean and standard deviation

Case study

Compare doses 3 and 4 mg/kg for adults. Which dose keeps minimum voriconazole concentration at steady state above MIC (1 mg/L) for >= 90% of subjects?

library(cowplot)

data_3mg <- e %>% 
  as_tibble %>% 
  bind_cols(idata) %>%
  mutate(amt = 3*BW, rate = 3*BW) %>%
  select(ID, everything())

#run simulation
sims_3mg <- modA2 %>% 
  mrgsim_d(data=data_3mg, delta = delta, end = end, obsonly=T, outvars = c("CP")) %>% 
  filter(row_number() != 1)

sims2_3mg <- sims_3mg %>%
  group_by(time) %>%
  mutate(loCP = lo05(CP),
         medCP = median(CP),
         hiCP = hi95(CP)) %>%
  ungroup() %>%
  filter(ID == first(ID))

#plot population predictions 
gp_3mg <- ggplot(data = sims2_3mg, aes(x=time)) + 
  geom_line(aes(y=medCP), col="black") +
  geom_ribbon(aes(ymin=loCP, ymax=hiCP), alpha = 0.5) +
  scale_y_continuous(trans = "log10", limits=c(0.3,7)) +
  labs(title="Population simulation (3 mg/kg)", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw() + 
  geom_hline(aes(yintercept = 1), lty=2) 

gp_4mg <- gp + 
  labs(title="Population simulation (4 mg/kg)", x="time (h)", y="Plasma concentration (mg/L)") +
  geom_hline(aes(yintercept = 1), lty=2) +
  scale_y_continuous(trans = "log10", limits=c(0.3,7))

gp_34 <- plot_grid(gp_3mg, gp_4mg, ncol=2) 
gp_34

# calculate stats
stats_4mg <- sims %>%
  filter(time != 0) %>%
  group_by(ID) %>%
  mutate(Cmin = min(CP)) %>%
  slice(1) %>%
  ungroup() %>%
  mutate(FLG = ifelse(Cmin < 1, 1, 0))
percHi_4mg <- (nrow(stats_4mg[stats_4mg$FLG==0,]) / nrow(stats_4mg)) * 100

stats_3mg <- sims_3mg %>%
  filter(time != 0) %>%
  group_by(ID) %>%
  mutate(Cmin = min(CP)) %>%
  slice(1) %>%
  ungroup() %>%
  mutate(FLG = ifelse(Cmin < 1, 1, 0))
percHi_3mg <- (nrow(stats_3mg[stats_3mg$FLG==0,]) / nrow(stats_3mg)) * 100 

stats_df <- tibble(Dose = c("3 mg/kg","4 mg/kg"),
                   `Percent of subjects with SS Cmin > MIC` = c(percHi_3mg, percHi_4mg))

stats_df %>%
  knitr::kable() %>%
  kable_styling()
Dose Percent of subjects with SS Cmin > MIC
3 mg/kg 71
4 mg/kg 96

iii. Parallelization

Use the package mrgsim.apply (vignette: https://github.com/kylebaron/mrgsim.parallel) to run the same population simulation over parallel cores. How long does it take compared to the single core simulation?

#Sys.setenv(R_LIBS = "../renv/library")
library(future)
library(mrgsim.parallel)

# set parallelization options
nCores <- future::availableCores()
options(future.fork.enable=TRUE, mc.cores = nCores)
plan(multicore, workers = nCores)

#run simulation
system.time(sims <- modA2 %>% 
  future_mrgsim_d(data=data, nchunk = 6L, delta = delta, end = end, obsonly=T, outvars = c("CP")) %>% 
  filter(row_number() != 1))
##    user  system elapsed 
##  11.905   0.164   5.069
# get summary stats for population
hi95 <- function(x) quantile(x, probs = c(0.95))
lo05 <- function(x) quantile(x, probs = c(0.05))

sims2 <- sims %>%
  group_by(time) %>%
  mutate(loCP = lo05(CP),
         medCP = median(CP),
         hiCP = hi95(CP)) %>%
  ungroup() %>%
  filter(ID == first(ID))

#plot population predictions 
gp <- ggplot(data = sims2, aes(x=time)) + 
  geom_line(aes(y=medCP), col="black") +
  geom_ribbon(aes(ymin=loCP, ymax=hiCP), alpha = 0.5) +
  scale_y_continuous(trans = "log10") +
  labs(title="Population simulation - parallel", x="time (h)", y="Plasma concentration (mg/L)") +
  theme_bw()
gp

g. Shiny - Interactive Document

Explore the simple shiny app saved as app.R

3. Monoclonal Antibody Model

Main differences between small and large molecule (mAb) PBPK models

  • mAb clearance is controlled by interaction with endosomal FcRn
  • mAbs typically don’t enter the intracellular space
  • Lymphatic system is crucial for mAb drainage to circulation

https://pubmed.ncbi.nlm.nih.gov/34687040/

Case study: Bamlanivimab

The story:

  • In early 2020, Lilly approached Metrum to collaborate on developing a novel mAb treatment for COVID-19.
  • The objectives were:
    • Select a mAb candidate from the candidate list
    • Select the dose for first-in-human (FIH) trials
  • What we had:
  • Method: Build a mAb PBPK model and use available in vitro data for different candidates to address the objectives.

OPEN SCIENCE

  • Results:
    • Identified bamlanivimab (LY-CoV555) as the best mAb candidate that would achieve the target concentration (IC90) in the lung tissue over 28 days in 90% of patients using the lowest dose possible.
    • The PBPK model predictions suggested a clinical dose between 175-500 mg. The viral dynamics model suggested a clinical dose of 700 mg (achieves maximum viral elimination).

  • Results validation after clinical data became available:

https://github.com/metrumresearchgroup/bioPBPK

mod_mab <- mread("models/mAb_bamlanivimab.mod")

# set up simulation conditions
dose <- 700/150 #700 mg to umol
dur <- 2
rate <- dose/dur
cmt <- 4
end <- 28*24
e <- ev(amt=dose, rate=rate, cmt=cmt)

sim_mab <- mod_mab %>%
  mrgsim_e(e, end=end, outvars=c("Cexg_Plasma", "Cexg_Lung_IS")) %>%
  as_tibble() 

sim_mab2 <- sim_mab %>%
  mutate(time = time/24,
         Plasma = Cexg_Plasma*150,
         `Lung interstitial` = Cexg_Lung_IS*150) %>%
  select(-Cexg_Plasma, -Cexg_Lung_IS) %>%
  gather(tissue, conc, -ID, -time)

# plot
p_mab <- ggplot(data=sim_mab2, aes(x=time, y=conc, col=tissue)) +
  geom_line(lwd=1) +
  geom_hline(yintercept = 0.41481, lty=2) +
  scale_x_continuous(breaks=seq(0, 28, 7)) +
  scale_y_continuous(trans = "log10") +
  labs(x="Time (d)", y=expression("mAb concentration ("*mu*"g/mL)")) +
  theme_bw() +
  theme(legend.text = element_text(size = 15),
        legend.title = element_text(size = 15),
        axis.text = element_text(size=15),
        axis.title = element_text(size=20),
        title=element_text(size=20))
p_mab